Anti-IFN gamma Receptor beta/AF-1/IFNGR2 Antibody Picoband™

Product Name

Anti-IFN gamma Receptor beta/AF-1/IFNGR2 Antibody Picoband™

View all IFN-gamma R2 Antibodies

SKU/Catalog Number

A03158-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-IFN gamma Receptor beta/AF-1/IFNGR2 Antibody Picoband™ catalog # A03158-2. Tested in ELISA, WB applications. This antibody reacts with Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IFN gamma Receptor beta/AF-1/IFNGR2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03158-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse IFN gamma Receptor beta/AF-1/IFNGR2 recombinant protein (Position: A20-P332).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03158-2 is reactive to IFNGR2 in Mouse

Applications

A03158-2 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

55 kDa

Calculated molecular weight

37.806kDa

Background of IFN-gamma R2

Interferon gamma receptor 2 also known as IFN-γR2 is a protein which in humans is encoded by the IFNGR2 gene. It is mapped to 16 C3.3; 16 53.07 cM. This gene (IFNGR2) encodes the non-ligand-binding beta chain of the gamma interferon receptor. Human interferon-gamma receptor is a heterodimer of IFNGR1 and IFNGR2. Defects in IFNGR2 are a cause of mendelian susceptibility to mycobacterial disease (MSMD), also known as familial disseminated atypical mycobacterial infection. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Mouse
Direct ELISA, 0.1-0.5μg/ml, Mouse

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of IFN Gamma Receptor Beta/AF-1/Ifngr2 using anti-IFN Gamma Receptor Beta/AF-1/Ifngr2 antibody (A03158-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: mouse thymus tissue lysates,
Lane 2: mouse lung tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFN Gamma Receptor Beta/AF-1/Ifngr2 antigen affinity purified polyclonal antibody (Catalog # A03158-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFN Gamma Receptor Beta/AF-1/Ifngr2 at approximately 55KD. The expected band size for IFN Gamma Receptor Beta/AF-1/Ifngr2 is at 38KD.

Gene/Protein Information For IFNGR2 (Source: Uniprot.org, NCBI)

Alternative Names

AF-1; AF-1interferon-gamma receptor beta chain precursor10IFNGT1Interferon gamma receptor accessory factor 1; IFGR2; IFN-gamma R2; IFN-gamma receptor 2; IFNgammaR2; IFN-gamma-R2; IFNGR2; IFN-gR2; IFNGT1; interferon gamma receptor 2 (interferon gamma transducer 1); interferon gamma receptor 2; interferon gamma receptor accessory factor-1; interferon gamma receptor beta chain; Interferon gamma transducer 1 IFNGR2 AF-1, IFGR2, IFNGT1, IMD28 interferon gamma receptor 2 interferon gamma receptor 2|IFN-gamma receptor 2|IFN-gamma-R-beta|IFN-gamma-R2|interferon gamma receptor accessory factor-1|interferon gamma receptor beta chain|interferon gamma transducer 1

For more info on IFNGR2, check out the IFNGR2 Infographic

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In this infographic, you will see the following information for IFNGR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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